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The expression profiles of NCK
2021-01-18

The expression profiles of NCK and ABI genes after ESC bacterial infection were determined. As shown in Fig. 1a, NCK1, ABI2a and ABI2b were significantly induced after ESC infection. While, ABI3a was significantly downregulated. Intestine was confirmed as a site of E. ictaluri entry by bacteriologic
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TgTCEA from petals TgTCEA and TgTCEB from
2021-01-18

TgTCEA from petals (TgTCEA1) and TgTCEB from pollen grains (TgTCEB1) were used throughout the study. The recombinant enzymes were expressed in , and purified as the His-tag-free forms as described previously., Enzyme reactions were performed at 4 mM substrates under the same conditions as described
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Methods br Results br Discussion In this
2021-01-18

Methods Results Discussion In this study we make the novel observation that villin-1 and gelsolin are down-regulated in response to diverse cellular stressors, including bacteria associated with the pathogenesis of CD. The high plasticity and dynamics of the Cy5 EGFP cytoskeleton are exploit
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We found that the postnatal absence of norepinephrine yields
2021-01-18

We found that the postnatal absence of norepinephrine yields differing effects depending on noradrenergic receptor type and Butaprost region. The density of α1-AR, indicated by [3H]prazosin binding was similar between Dbh+/− and Dbh−/− mice except for a significant increase in hippocampus in Dbh−/−
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In the present study DRD decreased
2021-01-18

In the present study, DRD3 decreased in the VTA of OA when compared with the control group. It is reported that DRD3 in the VTA control the firing rate of dopaminergic neurons and inhibition of these receptors enhance firing in the VTA-projecting neurons [18]. A study also indicated that morphine tr
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In conclusion we have recombinantly expressed and purified t
2021-01-18

In conclusion, we have recombinantly expressed and purified the product of the YP_002262821.1 gene from A. salmonicida and confirmed that it possesses ATP-dependent DNA ligase activity. Comparison of the full-length and truncated versions of the protein indicate that the N-terminal 25 dihydrofolate
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Other factors also contribute to replication fork
2021-01-18

Other factors also contribute to replication fork progression in DNA damage conditions. For instance, the cullin Rtt101 has a role in fork movement through alkylated DNA, probably associated to a damage tolerance mechanism [52], and TORC signaling is also important for fork progression in response t
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The focus of this review is on the diacylglycerol acyltransf
2021-01-18

The focus of this review is on the diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) enzymes, DGAT1 and DGAT2, that catalyze the esterification of a fatty acyl moiety to a DAG molecule to produce TAG. These enzymes commit DAG to being stored as TAG rather than being diverted for the synthesis of ph
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Both anxiogenic and antinociceptive effects
2021-01-18

Both anxiogenic and antinociceptive effects produced by CRF seem to be selective for the CRF1 receptor, since intra-dPAG microinjection of ASV 30, a CRF2 receptor antagonist, altered neither the anxiety nor the antinociception response. Intra-dPAG ASV 30 was also unable to prevent the anxiogenic (ob
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Increasing age among middle aged subjects
2021-01-15

Increasing age among middle-aged subjects (30–59years old) was associated with higher levels of cholesterol synthesis markers (although without statistical significance) and also of cholesterol Dexlansoprazole markers (with statistical significance for campesterol and sitosterol); this finding is c
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Acknowledgments The authors thank Dr Vladimir Poltoratsky an
2021-01-15

Acknowledgments The authors thank Dr. Vladimir Poltoratsky and Zhihui Xiao for their help with the in vitro FRET assay. Support for this research was provided by Saint John’s University. Introduction We predicted the existence of endothelium-derived contractile factors (EDCFs) in 1982 [1]. In 1
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Sampling sediments is usually performed
2021-01-15

Sampling sediments is usually performed with grab corers [16], [29]. The digested sludge analyzed by Ternes et al. [16] was taken directly from the digester and the activated sludge was obtained by D-Pantothenic acid of the slurry of STPs [16]. Collected samples are transported under cooled conditi
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The Acat gene was identified by functional complementation o
2021-01-15

The Acat1 gene was identified by functional complementation of a Chinese hamster ovary cell mutant lacking ACAT activity [12]. Unlike most other genes, human Acat1 is located in two different chromosomes, hcv protease inhibitors 1 and 7, with each site containing a distinct promoter: chromosome 1 co
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br Acknowledgments This work was supported
2021-01-15

Acknowledgments This work was supported by Région Auvergne “Nouveau chercheur”. This study has been performed with the assistance of Christelle Damon-Soubeyrand for histology technical assistance using “Anipath” Platform (GReD) and Sandrine Plantade, Keredine Ouchen and Philippe Mazuel for animal
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Crystallographic and NMR based analyses have revealed that R
2021-01-15

Crystallographic and NMR-based analyses have revealed that RINGs and U-boxes have a common mode of interaction with E2s (Fig. 2A). The key structural elements are two loop-like regions, which, in the case of RINGs, coordinate Zn. The loops surround a shallow groove formed by the central α-helix. Tog
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